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KMID : 1134820090380060801
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Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 6 p.801 ~ p.806
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Purification and Characterization of Transglutaminase from a Newly Isolated Streptomyces platensis YK-2
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Ko Hee-Sun
Kim Hyun-Su
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Abstract
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A species producing transglutaminase (EC 2.3.2.13) was isolated from forest soil and identified as Streptomyces platensis YK-2. The transglutaminase was purified from culture broth by 50% methanol precipitation, followed by successive chromatography on DEAE-Sephadex. The yield and purification-fold was 63.4% and 2.2-fold, respectively. The purified microbial transglutaminase (MTG) migrated as a single band of approximately 45 kDa upon sodium dodecyl sulfate polyacrylamide gel eletrophoresis. The isoelectric point determined by multichambered electrofocusing was pH 6.0~7.0. The enzyme was strongly inhibited by Hg??, but was activated by Cd??, Mg??, Mn??, Pb?? and reducing agents such as dithiothreitol and mercaptoethanol.
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KEYWORD
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microbial transglutaminase, Streptomyces platensis YK-2, purification, methanol precipitation, DEAESephadex
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